SOX-9 also plays a pivotal role in male sexual development; by working with Sf1, SOX-9 can produce AMH in Sertoli cells to inhibit the creation of a female reproductive system.  It also interacts with a few other genes to promote the development of male sexual organs. The process starts when the transcription factor Testis determining factor (encoded by the sex-determining region SRY of the Y chromosome ) activates SOX-9 activity by binding to an enhancer sequence upstream of the gene.  Next, Sox9 activates FGF9 and forms feedforward loops with FGF9  and PGD2 .  These loops are important for producing SOX-9; without these loops, SOX-9 would run out and the development of a female would almost certainly ensue. Activation of FGF9 by SOX-9 starts vital processes in male development, such as the creation of testis cords and the multiplication of Sertoli cells .  The association of SOX-9 and Dax1 actually creates Sertoli cells, another vital process in male development. 
Dehydroepiandrosterone comes into subsequent oxidative transformation with production of 16Alpha-hydroxydehydroepiandrosterone . This oxidation is catalyzed by Cytochrome P450, family 3, subfamily A, polypeptide 7 ( CYP3A7)  ,  ,  and Cytochrome P450, family 3, subfamily A, polypeptide 4 ( CYP3A4)  ,  ,  . Oxidative metabolite of this reaction as well as Dehydroepiandrosterone can be further sulfated by steroid sulfatase (microsomal), isozyme S ( STS )  ,  ,  ,  ,  . Dehydroepiandrosterone and Dehydroepiandrosterone sulfate can be transformed into other compounds with hormonal activity, Androstendiol and Androstendiol sulfate , respectively. These two reactions are catalyzed by Hydroxysteroid (17-beta) dehydrogenase 1 ( HSD17B1)  ,  ,  ,  , Hydroxysteroid (17-beta) dehydrogenase 2 ( HSD17B2)  ,  ,  ,  , and Hydroxysteroid (17-beta) dehydrogenase 7 ( HSD17B7 )  ,  ,  ,  .