Steroid-based facial amphiphiles for stabilization and crystallization of membrane proteins

Dr Raghunatha Reddy Organizing chairman mentioned “Another alarming consequence of such abuse is the emergence of resistance to commonly used antibiotics and antifungals rendering the treatment of infections more difficult and more expensive”. As required by law in India; strong steroids can be sold only with a registered medical practitioner’s prescription. However, topical steroids are exempt and can be purchased over the counter, which needs urgent revision. Another problem that leads to the inappropriate use of topical steroids is that too few specialist dermatologists are available.

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Weaker topical steroids are utilized for thin- skinned and sensitive areas, especially areas under occlusion, such as the armpit, groin, buttock crease, breast folds. Weaker steroids are used on the face, eyelids, diaper area, perianal skin, and intertrigo of the groin or body folds. Moderate steroids are used for atopic dermatitis , nummular eczema , xerotic eczema , lichen sclerosis et atrophicus of the vulva , scabies (after scabiecide) and severe dermatitis . Strong steroids are used for psoriasis , lichen planus , discoid lupus , chapped feet, lichen simplex chronicus , severe poison ivy exposure, alopecia areata , nummular eczema, and severe atopic dermatitis in adults. [1]

FIG. 2 illustrates: ( a ) SDS-PAGE showing purity of MsbA extracted from membranes and purified with β-UDM (lane 1) (positive control) and compound 1 (lane 2); ( b ) ATPase activity of freshly purified MsbA in β-UDM and compound 1; ( c ) MsbA stability in the presence of β-UDM (solid square) and compound 1 (open circle) at RT, examined by the ATPase activity as a function of time. The data is the average of 3 measurements with standard error bars shown. FIG. 2 ( d ) illustrates ATP hydrolysis by NBD (solid triangle) and MsbA (solid square, in compound 1), indicated by the decrease in absorbance of NADH at 340 nm. UDM is undecyl-β-D-maltoside.

Steroid-based facial amphiphiles for stabilization and crystallization of membrane proteins

steroid-based facial amphiphiles for stabilization and crystallization of membrane proteins

FIG. 2 illustrates: ( a ) SDS-PAGE showing purity of MsbA extracted from membranes and purified with β-UDM (lane 1) (positive control) and compound 1 (lane 2); ( b ) ATPase activity of freshly purified MsbA in β-UDM and compound 1; ( c ) MsbA stability in the presence of β-UDM (solid square) and compound 1 (open circle) at RT, examined by the ATPase activity as a function of time. The data is the average of 3 measurements with standard error bars shown. FIG. 2 ( d ) illustrates ATP hydrolysis by NBD (solid triangle) and MsbA (solid square, in compound 1), indicated by the decrease in absorbance of NADH at 340 nm. UDM is undecyl-β-D-maltoside.

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steroid-based facial amphiphiles for stabilization and crystallization of membrane proteins